Description
Description: This assay employs a two-site sandwich ELISA to quantitate KDM5D in samples. An antibody specific for KDM5D has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KDM5D present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KDM5D is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KDM5D bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: KDM5D encodes a protein containing zinc finger domains. A short peptide derived from this protein is a minor histocompatibility antigen which can lead to graft rejection of male donor cells in a female recipient. Alternative splicing results in multiple transcript variants.It was clustered with the Ube1y and the Zfy1 genes in a segment of approximately 250 kb. A homologous gene, Smcx (JARID1C), was found on the X chromosome. Expression of Smcy was detected in all male tissues and expression of Smcx in all male and female tissues tested. Remarkably, the expression of both genes was detected in pools of mouse preimplantation embryos as early as the 2-cell stage. They found that Smcy, like Sry and Ube1y, has been conserved on the Y chromosome since the divergence of metatherian and eutherian mammals some 120 million years ago